Fig. 2

Inhibition of BCAR4 on cell migration, invasion and DOX resistance in both MCF-7 and MDA-MB-231 cells. A and E RT-qPCR assay for BCAR4 expression in MCF-7 and MD-MB-231 cells transfected with BCAR4 siRNA and control. B, C, D, F, G and H Transwell assay for cell migration and invasion with inhibition of BCAR4. I and J Wound healing assay for cell migration with inhibition of BCAR4. K and L Colony formation of cells transfected with BCAR4 siRNA. M and N MCF-7 and MDA-MB-231 transfected with BCAR4 siRNA or control were treated with increasing doses of DOX. Cell proliferation and IC50 were detected by CCK8 assay (n = 5). The concentration of DOX (X axis) was presented on a logarithmic scale. Results were expressed as OD value as measured. O Western blot assay of the expression of ABCB1 and metastasis-related proteins, E-cadherin, Vimentin and VEGF in MCF-7 and MDA-MB-231 cells transfected with BCAR4 siRNA and control. Results were presented as mean ± SD of three independent experiments. *P < 0.05, ** P < 0.01, and  *** P < 0.001