Fig. 4

BCAR4 promotes migration, invasion and chemo-resistance of MCF-7 and MDA-MB-231 by downregulating miR-644a. A and B Colony formation of cells transfected with si-BCAR4 and/or miR-644a inhibitor. C and D Wound healing assay for migration of cells transfected with control, si-BCAR4 or the combination of both si-BCAR4 and miR-644a inhibitor. E and F Transwell assay for migration and invasion of cells transfected with control, si-BCAR4 or si-BCAR4 together with miR-644a inhibitor. G MDA-MB-231 transfected with si-BCAR4 and/or miR-644a inhibitor was treated with gradient doses of DOX. Cell viability and IC50 were detected by CCK8 assay (n = 5). The concentration of DOX (X axis) was presented on a logarithmic scale. Results were presented as the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. H Western blot assay of ABCB1, E-cadherin, Vimentin and VEGF in MDA-MB-231 transfected with si-BCAR4 and/or miR-644a inhibitor