Fig. 2

PDOs recapitulate genomic and transcriptomic profile of original tissues. (A) Concordance of SNVs and InDels between PDOs and corresponding tissues. On the left percentage of genomic alterations detected across the study subjects. (B) Whole Genome View representation of long-term PDOs and corresponding tissues according to Cytoscan HD. Data is expressed as the weighted log2 ratio of the copy number on the left Y-axis, and the chromosome number on the X-axis. 0 corresponds to diploid, upper and lower spikes indicate gain and loss regions, respectively. mCTO50 tissue was not available. (C) Heatmap showing the Pearson correlation coefficient (color key) between tumors (rows) and organoids (columns) based on the normalized counts as described in materials and methods section. Dendrograms show the hierarchical clustering based on the complete method and Euclidean pair-wise distance. Different passages from the same organoid culture are included