Fig. 1

The snRNA-seq analysis of the tumour tissues of patients with stage-I and II cervical cancer. (A) Experimental design: the tumour tissues were collected from the patients at The First Affiliated Hospital/School of Clinical Medicine of Guangdong Pharmaceutical University. (B) t-Stochastic neighbour embedding (t-SNE) representation of aligned gene expression data in single nuclei extracted from the TME of CCI and CCII patients shows partition into 22 distinct clusters. (C) The proportions of the 22 cell clusters in the CCI and CCII groups. (D) The distributions of cell cycle phases in the t-SNE space. (E) Selected enriched genes used for biological identification of each cluster and the top 5 DEGs of each cluster (scale: log-transformed gene expression). MΦ represents macrophage; Treg cell, regulatory T cell; NK cell, natural killer cell; NPC, neural progenitor cell; GMPC, granulocyte-monocyte progenitor cell; pDC, plasmacytoid dendritic cell (see Table S2 for the full list of all marker genes detected)