Fig. 5

The B cells and DCs in the TME of the CCI group exhibiting enhanced MHC class-I pathway. (A) The proportions of B cells and DC cells detected in the CCI and CCII groups. (B) Comparison of the average expression of the genes associated with antigen presentation in the CD141⁺CLEC9A⁺ and pDC populations, with respect to their percentage of expression. (C) The top 25 biological processes enriched in CD141⁺CLEC9A⁺ and pDC populations. (D) Comparison of the normalised expression of selected genes associated with antigen presenting processes in B cells and DCs in the two groups. Student’s t-test was used to evaluate the significance (*: P < 0.05, **: P < 0.01, and ****: P < 0.0001). (E) The hierarchical clustering of the top 60 DEGs identified in the B cell populations between the two groups