Fig. 1

The characteristics of LPPC with bound antibodies. A A cryo-TEM instrument was utilized to take images of samples of LPPC complexes (2 mg/ml). Representative images of LPPC complexes were shown (scale bars: 100 nm). B The Bradford assay was used to determine the maximum amount of mAb on LPPC. C The activities of OKT3 and 2C11 mAbs on LPPC were respectively evaluated the cell proliferation rate by the MTT assay. D The cell proliferation rate of naive or activated mice splenocytes under LPPC/Ab complexes treatment was determined by the MTT assay. A significant difference compared to the naive splenocytes group was indicated by * (P < 0.05), and comparison to the LPPC/CD3 group is indicated by # (P < 0.05). E The IL-2 mRNA expressions of splenocytes were determined by real-time quantitative PCR experiments. A significant difference was indicated by * (P < 0.05) and ** (P < 0.01). F The caspase 3 activity as an apoptosis indicator was determined by Ab staining. The fluorescent mean of the cell was shown from the expression of caspase 3