Fig. 3
The activities of the immuno-LPPC complex incubated with splenocytes. A The picture illustrated how to investigate LPPC/HLA/Ab complexes could react the mice splenocytes that were successfully immunized with YML-peptides. B-G LPPC adsorbed peptides-loaded HLA-A2 molecules with or without mAbs co-incubated splenocytes, and the splenocytes had been immunized with YML peptides (solid bar) or not (open bar). The activities of immuno-LPPC were determined by MTT assay for the cell proliferation rate or ELISA for the cytokine expression (IL-2, IFN-γ, IL-4, TGF-β, and IL-10). A significant difference compared with LPPC alone was indicated by * (P < 0.05), ** (P < 0.01), and *** (P < 0.001). A significant difference compared with the LPPC/HLA group was indicated by # (P < 0.05). Data were expressed as the mean ± SD (N = 6)