Fig. 9

RNA-seq analysis of the immunomodulation activities by LPPC/MP complex with different antibodies. AThe picture illustrated how to collect the RNA of mice splenocytes after different treatments. B and C. Heatmap of RNA-seq expression data showing the genes that were differentially regulated under different treatments. N: no-treatment; LM: twice LPPC/MP treatments; LMA1: once LPPC/MP/CD28 treatment; LMA2: twice LPPC/MP/CD28 treatments; LMS1: once LPPC/MP/CTLA4 treatment; LMS2: twice LPPC/MP/CTLA4 treatments. D A heatmap of 25 kinds of immune cells under the effects of different treatments, analyzed through the CIBERSORTx website, was shown. The speculated mechanisms and pathways were created using BioRender (https://biorender.com/). The figure showed how LPPC/MP/CD28 complex activated immune responses, according to RNA-seq analysis and related literature. E Chemokines and immune cells. F Cytokines and immune cells