Table 1 The particle sizes and surface charges of LPPC in combination with immunofunctional molecules. LPPC adsorbed different mAbs and proteins in the following experiments. The addition of PEG for charge neutralization was represented as w/, otherwise, it was shown as w/o. A Zetasizer was used to analyze the characteristics, particle size, and zeta-potential of LPPC with different molecules. All values were the mean ± SD of three independent experiments (N = 6)
Formulation | Size (nm) | Zeta-potential (mV) |
|---|---|---|
LPPC (w/o) | 184.2 ± 12.4 | 40.7 ± 5.1 |
LPPC (w/) | 208.4 ± 19.2 | 8.2 ± 4.5 |
LPPC/OKT3/CD28 (w/) | 221.7 ± 13.1 | 11.8 ± 4.1 |
LPPC/2C11/CD28 (w/) | 214.3 ± 21.2 | 9.7 ± 1.5 |
LPPC/MP (w/) | 255.9 ± 18.2 | 8.1 ± 3.1 |
LPPC/MP/CD28 (w/) | 275.0 ± 21.6 | 6.9 ± 4.4 |
LPPC/MP/CTLA4 (w/) | 264.1 ± 15.7 | 5.8 ± 2.9 |
LPPC/HLA/CD28 (w/) | 325.6 ± 25.1 | 10.8 ± 4.4 |
LPPC/HLA/CTLA4 (w/) | 314.1 ± 19.5 | 8.4 ± 3.6 |