Fig. 3

LARP6 up-regulates ZNF267 expression by binding and stablizing ZNF267 mRNA. A KEGG enrichment analysis of LARP6 using three different GEO datasets. B qPCR analysis of candidate genes in LARP6-overexpressed SW480 cells and control cells. C Correlation analysis of mRNA expression between LARP6 and ZNF267 in 49 paired fresh CRC tissues and matched adjacent normal tissues. D RIP-qPCR assay with anti-LARP6 antibody in SW620 cells (N = 3). GAPDH as a negative control. E RNA-pull down assay with ZNF267 RNA probe in CRC cells. Input represents 1% of lysate used in pulldown reactions. UN indicates a control pulldown containing beads only. F Affection of LARP6 on ZNF267 protein expression was analyzed by WB. G-J With a transcription inhibitor ActD, ZNF267 mRNA stability in CRC cells with LARP6 over-expression (G-H) or knockdown (I-J) was detected. K-N Sucrose gradient fractionation was conducted to analyze the influence of LARP6 on translation activity of ZNF267 mRNA. *P < 0.05, **P < 0.01, ***P < 0.001, ns means no statistic difference. The error bars represent Mean ± SD