Fig. 3
Pro suppresses viability and maturation of osteoclasts. A Cell viability of U937, RAW264.7 cells and primary bone marrow macrophages (BMMs) treated with gradient concentrations of Pro. IC50 values of Pro in each cell type are indicated on the graph. B Representative TRAP staining images of BMMs-derived osteoclasts stimulated by RANKL for 14 days treated with DMSO or Pro at indicated concentrations. The area of mature OCs was quantification on the right. C Representative images of bone resorption by BMMs-derived osteoclasts treated with DMSO or Pro at indicated concentrations. The area of absorbed bone was quantification on the right. D Fold change of mRNA level of osteoclast marker genes in BMMs in indicated conditions. *P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance