Fig. 4
Pro inhibits bone-metastatic PCa cells in vivo. A and B Representative images for Nude mice injected with C4-2B (A) and PC-3 (B) in tibias and treated with PBS or Pro. Bioluminescence was quantified on the right. C and D Representative 3D micro-CT reconstructions (C) and H&E staining (D) of C4-2B-involved tibias in PBS or Pro treated group. Yellow arrows indicate aberrant woven bone formation in the bone marrow. Scale bars are indicated in the lower right corner. E and F Representative 3D micro-CT reconstructions (E) and H&E staining (F) of PC-3-involved tibias in PBS or Pro treated group. Yellow arrows indicate the bone destruction caused by cancer cells. G and H Representative images of separated trabecular bones in tibias involved by C4-2B (G) and PC-3 (H) and treated with PBS or Pro. I and J Quantitative bone morphometric parameters of bone volume to total tissue volume (BV/TV), trabecular bone thickness (Tb.Th), trabecular number (Tb.N., mm−1) and trabecular spacing (Tb.Sp., mm) measured in bones involved by C4-2B (I) and PC-3 (J). K ALP staining in bone sections from C4-2B-involved tibias treated with PBS or Pro. L TRAP staining in bone sections from PC-3-involved tibias treated with PBS or Pro. *P < 0.05, **P < 0.01, *** P < 0.001, ns, no significance