Fig. 1

Flowchart of the whole work. In a cohort of 191 GC patients and 288 healthy controls, we conducted a questionnaire survey on lifestyle habits and dietary preferences. To obtain genetic information, peripheral blood leukocytes were collected from patients and controls and used for GWAS. After analysis of genetic and nongenetic risk factors, the DHCR7 gene and its mutation site rs104886038 were identified. Further molecular function verification was carried out in vivo and in vitro experiments. Combined with protein structure analysis, we identified and verified the key co-mutation sites of DHCR7, thereby obtaining potential intervention targets for GC treatment