Fig. 4

Overexpression of DHCR7 promotes the proliferation, invasion and migration of GC cells via cellular cholesterol biosynthesis. A Western blot analysis verified that DHCR7 was stably overexpressed in GC cell lines infected with DHCR7 overexpression lentivirus. B Results of EdU incorporation assays in control GC cells, DHCR7-overexpressing GC cells and DHCR7-overexpressing GC cells treated with TAM or AY 9944 for 24 h. C Results of colony formation assays in control GC cells, DHCR7-overexpressing GC cells and DHCR7-overexpressing GC cells treated with TAM or AY 9944 for 14 days. D Results of Transwell migration and invasion assays in control GC cells, DHCR7-overexpressing GC cells and DHCR7-overexpressing GC cells treated with TAM or AY 9944 for 24 h. E Cellular cholesterol level in control GC cells, DHCR7-overexpressing GC cells and DHCR7-overexpressing GC cells treated with TAM or AY 9944 for 24 h, as measured by Filipin III staining. F Cholesterol level in control GC cells, DHCR7-overexpressing GC cells and DHCR7-overexpressing GC cells treated with TAM or AY 9944 for 24 h. AGS were treated with TAM (3 μM) and AY 9944 (5 μM). MKN-28 were treated with TAM (5 μM) and AY 9944 (10 μM). HGC-27 were treated with TAM (5 μM) and AY 9944 (5 μM). The data are presented as the means ± SDs and calculated by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001