Fig. 1

Alterations and pharmacological inhibition of CDK4/6 in GC cell lines. A Percentage aberrations in cell cycle-related genes shown in the TCGA-STAD cohort (PanCancer Atlas). B Gene Expression Profiling Analysis data from the TCGA databases of TCGA-STAD dataset demonstrated elevated mRNA levels of CDK4 and CDK6 in GC compared with adjacent normal tissue. C Immunohistochemistry (IHC) staining of CDK4 and CDK6 in GC patients from The Human Protein Atlas. D Percentage aberrations and mRNA expression in cell cycle-related genes shown in the GC cell lines. E Dose-response curves for ABE in 13 GC cell lines. ABE was evaluated in 10 serially diluted doses and each dose was analyzed in technical quadruplicate in each biological replication. The relative cell number was determined using the CellTiter-GLo signal captured on the luminescent microplate reader. F Average IC50 values generated from dose-response curves for ABE