Fig. 1

Implication of HES1, a member of NOTCH signaling pathway, in ccRCC. a NOTCH signaling pathway-related gene mutations found in TCGA KIRC. b Heat map correlation analysis between NOTCH signaling pathway and the malignant features by taking advantage of TCGA KIRC. c Relationship between stroma score and NOTCH signaling pathway based on TCGA KIRC. d Relationship between gefitinib (left) or gemcitabine (right) sensitivity and NOTCH signaling pathway based on TCGA KIRC. e Western blot analysis. Whole cell lysates were prepared from 32 pairs of ccRCC tissues (T) and para-cancerous ones (N), and analyzed for HES1 by western blot. β-tubulin was used as a loading control. f Western blot analysis. Relative band intensity analysis of HES1 in 32 cases. g qPCR analysis of HES1 in 60 ccRCC tissues and 30 para-cancerous ones. h Expression analysis of HES1 in TCGA KIRC (left), and Kaplan–Meier analysis based on HES1 expression in TCGA KIRC cohort (right)