Fig. 7

ARNT drives neutrophil recruitment, function and the gut microbiota in anti-colorectal cancer treatment. A-F Mouse colorectal cancer induction for 80 days after the injection of azoxymethane (AOM) and the addition of dextran sulfate sodium salt (DSS) to the drinking water. Meanwhile, an ARNT inhibitor (GNF351, 5 mg/kg body weight) was i.p. injected weekly as indicated in A. The number of colonic tumors (B) and the tumor diameter (C) are summarized. The graph summarizes data from three independent experiments with fifteen mice per group (B-C). D Percentage of tumor-infiltrating neutrophils in the colon. E Tumor-infiltrating neutrophils were isolated and stimulated with LPS in vitro as indicated to detect NETs. The percentage of NETs-forming cells was quantified. F Suppressive assay of tumor-infiltrating neutrophils from tumor-bearing mice. Data from coculture with allogeneic CD4⁺ T cells with different ratio between neutrophils and T cells are shown. Representative of three independent experiments with three mice per group (D-F). G-I Mouse colorectal cancer induction for 80 days after the injection of AOM and the addition of DSS to the drinking water. Meanwhile, GNF351 (5 mg/kg body weight) was i.p. injected weekly, and the feces of WT mice were intragastrically administered to these mice every other day as indicated in G. The number of colonic tumors (H) and the tumor diameter (I) are summarized. The graph summarizes data from three independent experiments with sixteen mice (H-I). *, P < 0.05 and ***P < 0.001, compared with the indicated groups. n.s., not significant