Fig. 2

GC cells-generated L-KYN leads to NK cell death in the TME in vivo. A The schematic diagram to show the construction process of humanized CDX tumor model in hIL-15 NOG mice. B The size of CDX tumors from the mice of different groups, measured by a caliper at various time points. The pictures on the left show the harvested tumors in one representative experiment. C The flow cytometric results to show the percentage of the transferred hNK cells (defined as CD3⁻CD56⁺) in hCD45⁺ lymphocytes, which infiltrated in the CDX tumor tissues with or without L-KYN treatment injected in the paratumor sites. The pictures on the left side were representative results. D The proportion of FVD⁺ hNK cells infiltrated in the tumor tissues, detected by flow cytometry. The results were replicated in 3 independent experiments with n = 4 for each group (B - D). * p < 0.05, ** p < 0.01, *** p < 0.001