Fig. 3
From: NAD pool as an antitumor target against cancer stem cells in head and neck cancer
The reduction in NAMPT expression decreases stemness in HNSCC cell lines. A Analysis of the clone phenotypes (holoclones, meroclones and paraclones) formed by NAMPT CRISPRs and control cells in RPMI-2650 and Detroit-562 cell lines. In each solid barr, bottom part are holoclones, medium part are paraclones and upper part are meroclones. In Detroit, no meroclones were observed. B Tumorsphere assay from single-cell sorting in RPMI-2650 or the whole cell population in Detroit-562. Representative images of the tumorspheres formed are shown. C Tumorigenicity of tumorspheres in vivo. Tumorspheres from NAMPT CRISPRs and control cells were injected into xenografts (N = 4). Images of the tumors, the efficiency of tumor formation and the time required to appear are presented. D Analysis of cell migration in NAMPT CRISPRs and control cells by Boyden Chamber assay. Representative microscopic images are shown (20X). E Analysis of positive and negative CD10, CD184, CD19, CD133, CD166 and CD44 subpopulations in NAMPT CRISPRs and control cells by FACS. The mean ± standard deviation is represented in all cases. Statistical analysis was performed with Student’s t test (*p < 0.05; **p < 0.01; ***p < 0.001)