Fig. 3

Identification and validation of SH3PXD2B, SH3PXD2A and EFHD2 as miR-1-3p, miR-26a-1-3p and miR-487b-3p direct targets. a Schematic representation of putative binding sites for miR-1, miR-26a-1 and miR-487b in the 3’UTR of the SH3PXD2B, SH3PXD2A and EFHD2 genes, respectively; b) Firefly luciferase activity in recipient cells after transient co-transfection with Renilla luciferase reporter plasmid containing the wild-type (wt) or the indicated mutant (mut) sequences of miR-1-3p, miR-26a-1-3p and miR-487b-3p target sites, and the relative miRNA-mimic or ctrl-mimic. Results are expressed as fold activation relative to the basal activity of pGL3 empty control (ctrl); c) SH3PXD2B-, SH3PXD2A- and EFHD2-mRNA and related protein expression levels in the indicated IDH-wt glioma cells after the indicate miRNAs overexpression. All the values are reported as mean of at least three experiments. Error bars indicate the standard deviation. * = p ≤ 0.05, ** = p ≤ 0.01