Fig. 5

FUBP1 promoted DVL1 transcription by binding to its enhancer region. A Peason correlation analysis of FUBP1 and DVL1 in TCGA-GBM and CGGA-GBM datasets. B Relative expression of FUBP1 and DVL1 in DBTRG and U251 cells after knockdown or overexpression of FUBP1. C ChIP-seq of FUBP1 in U251 cells. Distribution of FUBP1-binding sequence around transcription start/end site was shown. The binding motif of FUBP1 was analyzed. D IGV visualization of FUBP1-binding site with DVL1 enhancer. E Graphic presentation of DVL1-full length (FL), DVL1-P3 wildtype and mutant luciferase vectors. F Dual-luciferase reporter assay detect the effect of FUBP1 on DVL1 transcription. G-H ChIP detected the binding domain of FUBP1 with DVL1-P3. I Dual-luciferase reporter assay revealed the N + C domain of FUBP1 could promote DVL1 transcription. J Relative expression of DVL1 in 293 T cells after transfection of different truncated FUBP1 domains. K The effect of different truncated FUBP1 domains on the activation of Wnt/β-catenin signaling in DBTRG and U251 cells. ** P < 0.01, *** P < 0.001, # P > 0.05