Fig. 7

circMMD sponged miR-15b-5p to increase FZD6 expression and activated Wnt/β-catenin pathway. A Dual-luciferase reporter assay explored the binding of miR-15b-5p or miR-424-5p with circMMD. B FISH assay explore the colocalization of circMMD and miR-15b-5p. Scale bar = 10 μm. C FZD6 was selected among overlapped genes among RNA-seq of circMMD, starBase-predicted target genes, and gene list of Wnt/β-catenin signaling. D The RNA level of FZD6 in grade II, grade III, and grade IV gliomas in TCGA and CGGA datasets. E The prognostic value of FZD6 in GBM samples of TCGA and CGGA datasets. F Dual-luciferase reporter assay explored the binding of miR-15b-5p with FZD6. G-H The expression of miR-15b-5p and FZD6 in DBTRG (G) and U251 (H) cells after transfection of miR-15b-5p inhibitor or mimics. I-J The expression of miR-15b-5p and FZD6 (I) and the change of Wnt/β-catenin signaling (J) after co-transfection of circMMD OE with miR-15b-5p mimics or or sh-circMMD with miR-15b-5p inhibitor in DBTRG and U251 cells. K Schematic illustration of this study that TTF-mediated reduction of circMMD suppressed Wnt/β-catenin signaling via FUBP1/FIR/DVL1 and miR-15b-5p/FZD6 pathways. * P < 0.05, ** P < 0.01, *** P < 0.001, # P > 0.05