Fig. 6
From: CLDN6 inhibits breast cancer metastasis through WIP-dependent actin cytoskeleton-mediated autophagy
CLDN6 interacts with JNK via the PDZ-binding motif. A IF analysis was performed to detect the co-localization of CLDN6 (green) with JNK (red) in MDA-MB-231/CLDN6 cells, zoomed-in image (right). Scale bar: 3 μm. B ImageJ was used for colocation analysis. Pearson correlation analysis showed that r > 0.5. Manders, colocation coefficient showed that M1 > 0.5 and M2 > 0.5. C The interaction of CLDN6 and JNK was detected by Co–IP assay in MDA-MB-231/CLDN6 cells. D Western blot showed levels of JNK/c-Jun pathway and WIP in MDA-MB-231 cells with or without CLDN6ΔPBM overexpression. Results were from three independent experiments. E The interaction of CLDN6 and JNK was detected by Co–IP assay in MDA-MB-231/CLDN6ΔPBM cells. F IF analysis was performed to detect the co-localization of CLDN6 (green) with JNK (red) in MDA-MB-231/CLDN6ΔPBM cells. Scale bar: 3 μm. G ImageJ was used for colocation analysis. Pearson correlation analysis showed that r < 0.5. Manders, colocation coefficient showed that M1 < 0.5 and M2 < 0.5. H The potential transcriptional binding site of c-Jun on the CLDN6 promoter, and was verified by ChIP assays. I-J JNK/c-Jun feedback regulates CLDN6 expression. I Western blot showed levels of CLDN6 protein in MCF-7/CLDN6 and MDA-MB-231/CLDN6 cells with or without c-Jun knockdown. J Western blot showed levels of CLDN6 protein in MCF-7/CLDN6 and MDA-MB-231/CLDN6 cells with or without JNK knockdown. Results were from three independent experiments. *P < 0.05 and ns: no significance