Fig. 4

Intra-nodal administration of low-dose anti-CTLA4 mAb can prevent distant metastasis. Bilaterally inoculated MXH10/Mo/lpr were administered 5 mg/kg anti-CTLA4 to tbLN on day 4 and 8 to one of the two tumor-bearing SiLN. A. Experimental outline B. Two sets of representative bioluminescent images: in vivo (upper panel) and ex vivo (lower panel). C. ex vivo luciferase activity of the SiLN, Liver and Lung. (Control, n = 4; tbLN, n = 7) D. Representative image of control and treated liver. Red circle: metastatic nodules. E. Incidence of liver and lung metastases (Control, n = 6; tbLN, n = 7) F. Survival curves of mice. (Control, n = 12; tbLN, n = 10). Statistical analyses were done using ANOVA with Tukey’s test or unpaired two-tailed student’s t-test. Log-rank (Mantel-Cox) test for survival curves. *P < 0.05, **P < 0.01, and ****P < 0.0001. Data are represented as means ± SEM