Fig. 2

Knockdown of FYN inhibited the proliferation of gastric cancer cells. A Expression of FYN in four strains of gastric cancer cells and normal stomach epithelial cells. B Knockdown of FYN expression in SGC7901 and MGC803 cells. C MTT assay confirmed that inhibition of FYN expression significantly inhibited the proliferation of SGC7901 and MGC803 cells. 5 × 10³ cells per well, N = 3, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant. D Softagar assay confirmed that knockdown of FYN significantly inhibited the clone formation ability of MGC803 and SGC7901 cells. 8 × 10³ cells per well, N = 3, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant. E In vivo experiments confirmed that inhibition of FYN significantly inhibited the growth rate of tumors. 1 × 10.⁷ cells per mouse, N = 6. Tumor size was measured according to the formula: TV (mm3) = length × width2 × 0.5. N = 6. The data are expressed as the means ± standard deviations. *p < 0.05, ns not significant (Student’s T-test)