Fig. 5

TOPK mediates FYN to promote gastric cancer proliferation and metastasis. A Overexpression of FYN in AGS cells. B CCK-8 assay for OD value of PCMV, OE-FYN and OE-FYN + OTS514 groups. 5 × 10³ cells per well, N = 4, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant. C Overexpression of TOPK-WT and TOPK-Y272F in MGC803 cells. D CCK-8 assay for OD value of PCMV, TOPK-WT and TOPK-Y272F groups. 5 × 10³ cells per well, N = 4, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant. E Transwell assay to determine the difference between PCMV, OE-FYN and OE-FYN + OTS514 groups. The number of invaded cells per well was quantified. scale bar, 100 μm. N = 5, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant. F Transwell assay to determine the difference between PCMV, TOPK-WT and TOPK-Y272F groups. The number of invaded cells per well was quantified. scale bar, 100 μm. N = 5, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant