Fig. 6

Validation of downstream substrate molecules screened by phosphoproteomics. A Knockdown of TOPK expression in AGS cells. B Heatmap of differentially phosphorylated proteins. C Summary of differentially phosphorylated proteins and loci. D Western blot validation of the differences in HSPB1 protein and phosphorylation levels in AGS shmock and shTOPK cells. E Diagram of TOPK-KO mice (By Figdraw). F HE staining of gastric tissues of WT and TOPK KO mice. G IHC of gastric tissues from WT and TOPK KO mice to verify the expression levels of TOPK, Ki67, HSPB1 and p-HSPB1 (ser15)