Fig. 7

HSPB1 acts as a downstream effector of FYN/TOPK and mediates its biological function. A Colocalization of FYN and HSPB1 was visualized by confocal microscope in MGC803 and SGC7901 cells. Cytoplasmic and nuclear staining of FYN and TOPK was mostly merged together. B Colocalization of TOPK and HSPB1 was visualized by confocal microscope in AGS cells. C-D CCK-8 assay detected cell viability in Control group, shFYN group, shFYN + OE-TOPK group and shFYN + OE-TOPK + HSPB1 inhibitor J2 group. 5 × 10³ cells per well, N = 3, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant. E Representative images of migration and invasion of Control group, shFYN group, shFYN + OE-TOPK group and shFYN + OE-TOPK + HSPB1 inhibitor J2 group in MGC803 cells. F Statistical results of the number of migrated cells in each group of MGC803 cells. The number of invaded cells per well was quantified. scale bar, 100 μm. N = 5, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant. G Statistical results of the number of invasive cells in each group of MGC803 cells. The number of invaded cells per well was quantified. scale bar, 100 μm. N = 5, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001,ns not significant. H Representative images of migration and invasion of Control group, shFYN group, shFYN + OE-TOPK group and shFYN + OE-TOPK + HSPB1 inhibitor J2 group in SGC7901 cells. I Statistical results of the number of migrated cells in each group of SGC7901 cells. The number of invaded cells per well was quantified. scale bar, 100 μm. N = 5, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant. J. Statistical results of the number of invasive cells in each group of SGC7901 cells. The number of invaded cells per well was quantified. scale bar, 100 μm. N = 5, The data are expressed as the means ± standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant