Fig. 5

SEC63 promotes HCC cell metastasis relying on ACLY. A Migration assay or invasion assay was performed as indicated. Migration or invasion rate was further quantified (n = 3). B Migration assay or invasion assay was performed in SEC63-depleted HCC cells. Migration or invasion rate was further quantified (n = 3). C Wound healing assay was performed as indicated. Wound closure rate was quantified (n = 3). D Wound healing assay was performed in SEC63-depleted or Ctrl HCC cells. Wound closure rate was quantified (n = 3). E Huh7 cells without (shCtrl) or with SEC63 knockdown (shSEC63) were injected into BALB/c nude mice to establish a lung metastasis model (n = 6). F Huh7 cells were injected as indicated into BALB/c nude mice to establish a lung metastasis model. Moreover, the mice were treated with vehicle or ETC1002 (30 mg/kg) per 2 days for 8 weeks (n = 6). The data are expressed as the mean ± SD