Fig. 2

eEF2 was a potent target in ESCC. A eEF2 protein levels in knockdown cell lines. B Cell viability of KYSE140, KYSE450 and KYSE510 after knockdown of eEF2. C Colony formation of ESCC cell lines after knockdown of eEF2. D Cell viability and colony formation of KYSE410 after eEF2 overexpressed. E Cell viability and colony formation of KYSE410 after eEF2 fragment F363-858 overexpressed. F&G The tumor volumes, representative pictures, average body weight and tumor weights of LEG107 and LEG244 PDX cases after infected by eEF2 shRNA virus. H Immunohistochemical staining of ki67 in tumor slices. I Ki67 expression level in (H). J eEF2 protein levels after knocking down of eEF2 in PDX models. Student’s unpaired t test in (B, C, D, E, F, G, I). Error bars represent the mean ± SD