Fig. 5

eEF2 regulates the protein biosynthesis of TOP1 and TOP2. A Represent pictures and analysis of protein biosynthesis levels after TSN treatment. B left: the merged gene of three replicates RIP-sequence results; right: the enriched KEGG pathways of the merged genes. C The motifs of eEF2 targeted on TOP1 and TOP2. D The enriched peak levels of TOP1 and TOP2 in the RIP-Sequence. E IB, immunoprecipitation for eEF2; RNA, TOP1 and TOP2 mRNA levels after co-immunoprecipitation with eEF2; input, Western blot for the protein levels of eEF2. F The protein expression levels and correlation between eEF2 with TOP1 and TOP2 in ESCC cell lines and SHEE cell. G TOP1 and TOP2 protein levels after knockdown of eEF2. H Protein levels of TOP1 and TOP2 after treating by TSN. Statistical analysis was performed using Student’s unpaired t test in (A). Spearman’s nonparametric correlation test performed in (F). Error bars represent the mean ± SD