Fig. 2

MiR-371b-5p is regulated by LINC00183. A LINC00183 capable of regulating miRNAs was predicted by bioinformatic tool miRDB. B The relative expression score (miRNAs level/LINC00183 level) was calculated in 9 predicted miRNAs. C The expression of miR-371b-5p in T-LBL cells transfected by LINC00183 and shLINC00183. D Correlation between LINC00183 gene expression and miR-371b-5p level in the SYSUCC and AHAMU dataset. E The predicted binding sites between miR-371b-5p and LINC00183. The mutated site (LINC00183-mut) was used for luciferase reporter assays. The relative luciferase activities were detected in 293T cells transfected by LINC00183-WT and LINC00183-mut. F LINC00183 is abundant in the cytoplasm of Jurkat and SUP-T1 cells. U2 and actin were used as positive control. G Cytoplasmic enrichment of LINC00183 in T-LBL tissues. H RNA-IP was used to identify inhibition of miR-371b-5p by LINC00183. The expression levels of LINC00183 and miR-371b-5p were detected using quantitative RT-PCR. LINC00183, ectopic LINC00183 expression in T-LBL cells. shLINC00183, T-LBL cells were transfected by shRNA targeting LINC00183. Dox, doxorubicin. *, P < 0.05