Fig. 3

miR-371b-5p suppresses T-LBL progression and chemoresistance in vivo and in vitro. A Kaplan-Meier curves of progression-free survival (RFS) and OS stratified by miR-371b-5p expression in patients from the SYSUCC and AHAMU datasets. B MTT assay using T-LBL cells transfected by LINC00183, LINC00183 + miR-371b-5p, shLINC00183, shLINC00183 + anti-miR-371b-5p treated with Dox (100ng/ml). C The protein levels of Bax, Bcl-2 in SUP-T1 cells transfected by LINC00183, and LINC00183 + miR-371b-5p treated with Dox (100ng/ml). D Flow cytometric analysis of T-LBL cells transfected by LINC00183, shLINC00183, LINC00183 + miR-371b-5p, and shLINC00183 + anti-miR-371b-5p treated with Dox (100ng/ml). E The tumor growth curve of mouse xenografts. The xenografts were formed SUP-T1-LINC00183 or SUP-T1-LINC00183 + miR-371b-5p cells treated with Dox (1 mg/kg/time, 3 times/week). F The tumor size of mouse xenografts. G The protein expression of Bax, Bcl-2, and cleaved-caspase-3 in xenograft tissues. 3 xenografts tissues for each group. LINC00183, ectopic LINC00183 expression in T-LBL cells. shLINC00183, T-LBL cells were transfected by shRNA targeting LINC00183. LINC00183 + miR-371b-5p, T-LBL cells were transfected with LINC00183 and miR-371b-5p. shLINC00183 + anti-miR-371b-5p, T-LBL cells were transfected with shLINC00183 and anti-miR-371b-5p. Dox, doxorubicin. *, P < 0.05