Fig. 2

YAP, TP63 and TEADs associate and show common genomic binding regions in HBECs. a Venn diagram shows peak overlaps between YAP, TEAD and TP63 chromatin binding domains in HBECs. b Distribution of YAP/TEAD/TP63 ChIP-seq signal around ± 2 kb of YAP and YAP/TEAD overlapped peak regions (N = 4817 and 735). c Top transcription factor binding motifs enriched in the YAP/TEAD overlapped peak regions in HBECs. P-values were calculated by HOMER. d YAP, TEAD and TP63 ChIP-seq tracks shows the co-binding at the promoter regions of Hippo or TP63 canonical target genes. Overlapped peak regions are highlighted with red strips below. e Proximity ligation assays (PLA) demonstrating close association between YAP, TEAD and TP63 in proliferating HBECs. Representative images of association between the respective factors are shown, along with quantification showing the average number of PLA foci per cell across a field of view. The average of five fields of view (FOV) for each condition with standard error of mean is depicted. Unpaired t-test **p < 0.01, *** p < 0.001, ****p < 0.0001. f Western blot showing TEAD and TP63 co-immunoprecipitation in HBECS