Fig. 7

Cyst(e)inase increases cytotoxic T cells, reduces immunosuppressive myeloid cell populations and produces synergistic inhibition of HMVP2 allograft tumor growth. A, Allografted HMVP2 prostate tumors in syngeneic FVB mice treated with vehicle control or Cyst(e)inase (100 mg/kg, 2x/week, i.p.) for 2 weeks. CD4 + T-cells; CD8 + T-cells; CD8 + GranzymeB + T-cells; CD11b + gr1 + myeloid cells in the tumor (Students t-test). B, Growth of allografted HMVP2 prostate tumors in male syngeneic FVB mice. Data represents Mean ± SEM of both flank tumors in group of mice treated with vehicle control (n = 14), Cyst(e)inase (n = 16), Anti PDL1 (n = 14), or Cyst(e)inase + Anti PDL1 (n = 13). Two-Way repeated measure ANOVA followed by Bonferroni’s multiple comparison test. P < 0.05, ϕ compared to control; # compared to Cyst(e)inase and $, compared to Anti PDL1. C, Average body weight per mouse from allograft tumor study (B)