Fig. 2

Hepatic Ufl1 or Ufbp1 deficiency increases the susceptibility to HFD-induced fatty liver. A Immunostaining of α-SMA in liver tissues extracted from Ufl1^Δ/Δhep, Ufbp1^Δ/Δhep, and control mice. Quantitative data are shown in the right panel (n = 5 mice/group). B Western blot analysis of collagen I protein levels in liver tissues extracted from Ufl1^Δ/Δhep, Ufbp1^Δ/Δhep, and control mice. C Anatomic illustration of liver tissues extracted from Ufl1^Δ/Δhep, Ufbp1^Δ/Δhep, mice and control mice after 12 weeks of HFD feeding. D Changes in liver weight in Ufl1^Δ/Δhep, Ufbp1^Δ/Δhep, and control mice after 12 weeks of HFD feeding (n = 5 mice/group). E, F Assessment of liver injury in Ufl1^Δ/Δhep, Ufbp1^Δ/Δhep, and control mice through serum ALT and AST levels (n = 5 mice/group). G, H Detection of liver injury and fibrosis levels in Ufl1^Δ/Δhep, Ufbp1.^Δ/Δhep, and control mice using four different staining approaches. HE, Masson, and Sirius Red staining were used to determine the extent of liver fibrosis, and Oil Red O staining was used to detect adipocyte lipid droplets. Quantitative data are shown in the lower panels (n = 5 mice/group). * p < 0.05; ** p < 0.01; *** p < 0.001