Fig. 1

Ferroptosis inducer erastin downregulates SNAI3-AS1 expression by increasing DNA methylation level of its promoter. A The expression of eight candidate lncRNAs in U87MG, U251 and A172 cells under erastin (10 μM, 48 h) treatments as measured by RT-qPCR. B Dual-luciferase reporter assays showed the transcription activity of SNAI3-AS1 promoter after erastin (5/10/20 μM, 48 h) treatments. C The correlation between DNA methylation level and gene expression of SNAI3-AS1 according to cBioPortal database. D The expression of SNAI3-AS1 in U87MG, U251 and A172 cells under 5-AZA (1 μM, 24/48 h) treatments as measured by RT-qPCR. E A Prediction analysis of CpG islands in the sequence range of 3100 bp upstream from the transcriptional start site in the SNAI3-AS1 promoter region. F BSP results of SNAI3-AS1 methylation status in U87MG, U251 and A172 cells under erastin (5/10/20 μM, 48 h) treatments. G Heat map of methylation percentage of SNAI3-AS1 in U87MG, U251 and A172 cells under erastin (5/10/20 μM, 48 h) treatments. H The expression of SNAI3-AS1 in U87MG, U251 and A172 cells under erastin (10 μM, 48 h) or erastin (10 μM, 48 h) combined with 5-AZA (1 μM, 48 h) treatments as measured by RT-qPCR and agarose gel image. *P < 0.05, **P < 0.01, ***P < 0.001, and n.s., not significant