Fig. 4

SNAI3-AS1 inhibits mRNA stability of Nrf2. A A Venn Diagram showed the intersection result of correlation analysis between SNAI3-AS1 and ferroptosis-related genes in TCGA and CGGA693 databases. B RT-qPCR was used to detect the mRNA level of Nrf2 after SNAI3-AS1 overexpression or knockdown under DMSO and erastin (10 μM, 48 h) treatments. C The correlation between SNAI3-AS1 and Nrf2 in TCGA and CGGA693 databases. D Western blotting showed the level of Nrf2, GPX4 and 4-HNE after SNAI3-AS1 overexpression or knockdown under DMSO and erastin (10 μM, 48 h) treatments. E U87MG cells with SNAI3-AS1 overexpression and A172 cells with SNAI3-AS1 knockdown were transfected with a dual luciferase reporter plasmid containing Nrf2 promoter. The relative luciferase activity was measured and normalized. F After actinomycin D (5 μg/ml) treatment for 0, 2, 4, 6 h, RT-qPCR was used to analysis the Nrf2 mRNA stability in U87MG cells with SNAI3-AS1 overexpression and A172 cells with SNAI3-AS1 knockdown. G U87MG cells with SNAI3-AS1 overexpression and A172 cells with SNAI3-AS1 knockdown were transfected with a dual luciferase reporter plasmid containing Nrf2 mRNA 3’UTR. The relative luciferase activity was measured and normalized. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and n.s., not significant