Fig. 3

MiR-92a-1-5p directly target MAPK1. A Mapk1 expression determined using qPCR. B Western blotting of MAPK1 and pMAPK1 in Raw264.7 cells with stable overexpression of miR-92a-1-5p. GAPDH: internal control. C Immunofluorescence labeling of MAPK2/MAPK1 in Raw264.7 cells with stable overexpression of miR-92a-1-5p. The mean fluorescence intensity was decreased. D Schematic diagram of the wild and mutant UTRs used. E Detection of relative fluorescence intensity at 48 h post co-transfection. Data were analyzed using t test. *, P < 0.05; ***, P < 0.001