Fig. 4

The regulation glycolysis and cell proliferation of nanoparticles in CT26 cells. (A) The cytotoxicity test of nanoparticles. (B) The cytotoxicity test of SHK@HA-MPDA in NCM460 cells. (C) The cytotoxicity test of MPDA in CT26 cells. (D) and (E) Quantitative of exocellular lactate and intracellular ATP of CT26 cells treated with nanoparticles. (F) and (G) The expression and grayscale analysis of PKM2 and c-Myc in CT26 cells after treatment by nanoparticles. (H) The Schematic mechanism of the nanoparticles regulating glycolysis and proliferation. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001