Fig. 6

The crosstalk between EMT and glycolysis. (A) After treatment with nanoparticles, the representative images of colony-forming cells of CT26 cells and the statistical analysis of cell clusters in (E). (B) The representative images of scratch assay of CT26 cells and the statistical analysis of distance in (F). The representative images of migration (C) and invasion (D) of CT26 cells after suffering nanoparticles. (G) Expression levels of epithelial or mesenchymal markers in CT26 cells were analyzed by western blotting. (H) Expression levels of epithelial or mesenchymal markers of CT26 cells after suffering SHK@HA-MPDA but intervening with or without LA or SB431542 were analyzed by western blotting (LA: 10 mM; SB431542: 5 µM). (I) The schematic of the mechanism between EMT and glycolysis in mesenchymal-like cells and epithelial-like cells. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001