Fig. 6

DAC treatment reactivates p16 and suppresses tumor growth in colon tumor organoids. A Images of colon tumor organoids derived from Apc^Min/+; p16^cis/cis mice after treatment with DAC at the concentrations shown on days 3 and 5. B A dose-dependent decrease in proliferation was observed in tumor organoids treated with DAC. C Low-dose DAC (0.5 μM) was sufficient to induce p16 promoter demethylation and gene reactivation. Error bars represent SEMs of three replicate experiments. Mean values were compared between the 0.5 μM DAC treatment and control (PBS) treatment groups. P values were determined by a two-tailed Student’s t-test