Fig. 3
Kremen2 promotes NSCLC cell metastasis in vivo and in vitro. A The migration ability of A549-Krm2KO + Flag-Krm2 cells was detected by the Transwell assay. B and C Transwell migration assays were carried out after the Kremen2-knockdown of H1703 (B) and A549 (C) cells. Representative images and corresponding statistical analysis of the number of migrating cells. D–G Cellular migration and growth were analyzed by the scratch assay in A549 (D) and H1703 (E) cells. Corresponding statistical analysis of the number of migrating cells (F, G). H, I Representative images (H) and H&E staining (I) of the lungs from mice transplanted with A549-Krm2KO cells or A549-Krm2KO + Krm2 cells (n = 4) (scale bars: 100 μm). J The number of metastatic nodules per lung was measured. K Schematic diagram showing construction of the tail vein lung metastasis model using A549 cells with shCtrl-GFP or shKrm2-GFP. L Comparison of the GFP fluorescence signal intensity in shCtrl and shKrm2 cells. M, N Representative images (M) and H&E staining (N) of the lungs from mice transplanted with shCtrl-GFP or shKrm2-GFP cells (n = 4) (scale bars: 100 μm). O Fluorescence luminescence imaging of lung metastases. Fluorescence signal intensity histogram (lower panel). Bars indicate mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001