Fig. 4

The inhibition of Kremen2 is involved in several cancer-related signaling pathways. A Hierarchical clustered heatmap displaying the differentially expressed genes. B The KEGG pathway analysis reveals significantly enriched signaling pathways in the differentially expressed genes. C GO biological processes show the Z-score normalized expression of the differentially expressed genes in H1703 cells after Kremen2-knockdown according to the RNA-seq data, which were mainly enriched in biological processes, such as cell proliferation and migration. D, E Protein levels of Wnt and PI3K/AKT signaling as indicated in the H1703 cell line with Kremen2 knockdown were detected by western blot. F–H Effects of Kremen2 level on the expression of proteins associated with cell proliferation and the JAK2-STAT3 signaling pathway in A549 and H1703 cells