Fig. 5

Kremen2 promotes NSCLC cell proliferation by promoting the stability of EGFR. A EGFR levels in Kremen2 knockdown cells treated with siRNA. B EGFR levels in Kremen2 over-expressing and Kremen2 knockdown of A549 and H1703 cells. C EGFR and phosphorylated STAT3 (Y705) protein levels in A549-Krm2^KO and A549-Krm2^KO + Flag-Krm2 cells. D A549 cells stably expressing Kremen2 shRNA or over-expressing Kremen2 (OE-Krm2) were treated with MG132 or not. The cells were lysed, and western blotting was performed with the indicated antibodies. E A549 and H1703 cells transfected with siKrm2 small-interfering RNA were stimulated with or without EGF (50 ng/mL) for 15 min. F, G Cell proliferation was measured by the EdU proliferation assay (F) and the EGFR expression levels were measured in Kremen2 over-expressing A549 cells treated with siNC or siEGFR (G). H, I Cell proliferation was measured by the EdU proliferation assay (H) and EGFR levels were measured in cells transfected with or without the His-EGFR plasmid (I)