Fig. 2
From: Targeting PHB1 to inhibit castration-resistant prostate cancer progression in vitro and in vivo
PHB1 promotes castration resistance. After CSS culture for 3 d, LNCaP cells were transfected with pENTER/pENTER-PHB1, C4-2B, 22Rv1, and PC-3 cells were transfected with siNC/siPHB1, respectively. A Cell proliferation was determined by MTS assay. The absorbance was read at 490 nm at the indicated time points and normalized to 0 h values. ***P < 0.001. B Invasion and migration capacity of PCa cells were determined by transwell assay. Left panel: representative images; Right panel: quantitative results of triplicate experiments. *P < 0.05, **P < 0.01, ***P < 0.001. C Cell clonogenic ability was determined by plate colony formation assay. Left panel: representative images; Right panel: quantitative results of triplicate experiments. *P < 0.05, **P < 0.01. CSS Charcoal-stripped serum. h Hours