Fig. 6

GAA promotes transcriptionally active histone modifications at metastasis related genes. A-B RT-qPCR showing Myc RNA degradation rate after exogenous GAA treatment. Actinomycin D (Act D) was used to inhibit the DNA-dependent RNA polymerase activity. C Western blot showing the expression of active and repressive histone modifications at the cellular level. D The MA plot showing concentration of differential H3K4me3 peak between si-GATM and si-NC or si-GATM + GAA and si-GATM. The degree of color represents the P value. E The annotated distribution of differential H3K4me3 peaks on the gene functional elements. F-G The GO enrichment analysis of differential H3K4me3 peaks-annotated genes. H The Venn analysis of differential expressed genes and differential peaks-related genes. I Histone CUT&RUN tracks showing the enrichment peaks of H3K4me3 at metastasis-related genes. J ChIP-qPCR showing the level of H3K4me3 enriched to the promoters of identified metastasis-related genes and Myc, normalized to IgG group. K RT-qPCR showing the mRNA expression of metastasis-related genes. Data in (A-B, J-K) are presented as mean ± SEM by ordinary one-way ANOVA (Tukey’s multiple comparisons test). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001