Fig. 7

GAA promotes H3K4me3 of metastasis-related genes by upregulating histone methyltransferases expression. A Quantitative measurement of SAM in PANC-1-IN cells by LC–MS/MS. B The expression Heatmap of HMTs and HDMs. The color degree represents the relative expression level. C RT-qPCR showing the expression of different methyltransferases and demethylases of H3K4 after GATM knockdown and exogenous GAA (1 mM) treatment. D-E CUT&RUN experiment showing levels of H3K4me3 and H3K4me1 enriched to Myc promoter after knockdown of different methyltransferases of H3K4. F Western blot showing the expression of HMTs in PANC-1-IN cells. G GSE71729 data analysis showing the levels of epigenetic enzymes expression among normal pancreas, primary PDAC and metastasis of PDAC. H Kaplan–Meier survival analysis by using the median cutoff value of KMT2D expression by using GSE62452 dataset. I The IC50 curve of WDR5-0103 in PANC-1-IN cells. J Transwell migration assay of pancreatic cancer cells after intervention. K Western blot showing the expression of H3K4me3 after GAA and WDR5-0103 treatment. L CUT&RUN analysis showing the H3K4me3 enriched to the promoters of metastasis-related genes. M RT-qPCR showing the mRNA expression of metastasis-related genes. Data in (A, C-E, J, L-M) are presented as mean ± SEM by ordinary one-way ANOVA (Tukey’s multiple comparisons test). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001