Fig. 2

Overexpression of circPAPD4 inhibits proliferation and promotes apoptosis for BC cells. (A) After transduced with circPAPD4 overexpression vector (OE-circPAPD4) or empty vector (EV), circPAPD4 and linear PAPD4 mRNA expression in MCF-7 and SKBR3 cells were analyzed by RT-qPCR. (B-C) CCK-8 and EdU assays were used to measure proliferation ability of BC cells in indicated groups; Scale bar: 20 μm. (D-E) TUNEL and flow cytometry analyses were applied to evaluate the apoptosis of BC cells in indicated groups; Scale bar: 20 μm. (F) The expression of cyclin-D1, cyclin-E1, BCL-2, and BAX in indicated groups were evaluated by western blotting. (G) Tumor volume of BALB/c nude mice was inoculated with BC cells stably transduced with OE-circPAPD4 or EV. n = 3 mice each group. Data are presented as means ± SD. **p<0.01; ***p < 0.001.