Fig. 3

ADAR1 regulates the biogenesis of circPAPD4 via binding to the flanking inverted complementary sequences. (A) Diagram indicated the construction of four types of vectors (#1, #2, #3, and #4) with or without the flanking inverted complementary sequences. (B) Relative circPAPD4 expression was assessed by RT-qPCR in MCF-7 and SKBR3 cells transfected with four types of vectors or NC. (C) RT-qPCR was used to assess relative ADAR1 and circPAPD4 expression in BC cells transfected with the sh-ADAR1-1, sh-ADAR1-2, or sh-NC. (D-E) Anti-ADAR1 antibody was used to performed RNA immunoprecipitation for assessment of endogenous ADAR1 binding to RNA in BC cells. RT-qPCR was used to evaluate the combining relationship between ADAR1 and I1RCM/I4RCM. (F) The correlation between ADAR1 mRNA and circPAPD4 expression in 50 BC tissues was measured using Pearson’s Correlation analysis. Data were presented as means ± SD. ***p < 0.001.